| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 171 | Priestia megaterium | GCAGTTCATGCGGACGAA | TACGCGTATTCCACCGTCG | 58.44 | 59.94 | 234 | 104 |
100.00%
|
100.00%
|
| Primer 172 | Priestia megaterium | CGAGAACACCGGACGAAGA | TACGCGTATTCCACCGTCG | 59.42 | 59.94 | 263 | 104 |
100.00%
|
100.00%
|
| Primer 173 | Priestia megaterium | CAGCTACAACGCATGGTGG | ACCTAGCGCTTGGTCGTTC | 59.20 | 60.08 | 194 | 103 |
100.00%
|
100.00%
|
| Primer 174 | Priestia megaterium | ATGAGTCCCCAGCAGCTTG | TGGCCTCAGTAAGCTGCTG | 59.70 | 59.70 | 157 | 103 |
100.00%
|
100.00%
|
| Primer 175 | Priestia megaterium | TGCCGTGCACAGCTTAGT | AGTGAGGCTTCAGCGTTCC | 59.57 | 60.00 | 245 | 103 |
100.00%
|
100.00%
|
| Primer 176 | Priestia megaterium | TGCCGTGCACAGCTTAGT | TGAGGCTTCAGCGTTCCTG | 59.57 | 60.00 | 243 | 103 |
100.00%
|
100.00%
|
| Primer 177 | Priestia megaterium | TGCCGTGCACAGCTTAGT | AGGCTTCAGCGTTCCTGT | 59.57 | 58.85 | 241 | 103 |
100.00%
|
100.00%
|
| Primer 178 | Priestia megaterium | CGGAAGTTCGCGAAGTAGC | GGACCCATTCCACGTGGTA | 59.01 | 59.02 | 229 | 103 |
100.00%
|
100.00%
|
| Primer 179 | Priestia megaterium | TGTGCGCAAACCAATGCC | AACGGAGAATCGGCTGACG | 59.97 | 60.15 | 210 | 102 |
100.00%
|
100.00%
|
| Primer 180 | Priestia megaterium | ATGCCCTGACGGTCATGTT | ATCGAAGAGGCAGAGGGCA | 59.31 | 60.69 | 290 | 102 |
100.00%
|
100.00%
|